Journal: Cardiovascular Diabetology
Article Title: Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus
doi: 10.1186/s12933-022-01497-6
Figure Lengend Snippet: Tie2 + monocyte gating and quantification. Representative fluorescence-activated cell sorting plots and gating strategy for the quantification of Tie2 + monocytes in healthy control individuals ( a ) and individuals with type 2 diabetes mellitus (T2DM) ( b ). Left 4 panels: Gating of Tie2 + monocytes within each monocyte subset, based on isotype controls. Right panel: Graph of Tie2 + monocyte frequency within the respective monocyte subset. No differences in Tie2 + monocyte frequency within subsets were observed in healthy control individuals. Within individuals with T2DM, the percentage of Tie2 + cells was significantly higher within the intermediate monocyte subset compared to the other monocyte populations. The percentage of Tie2 + monocytes within the classical monocyte population was similar between healthy individuals and individuals with T2DM ( c ). The percentage of Tie2 + cells within in the intermediate monocyte population was 1.9-fold higher in individuals with T2DM compared to healthy control individuals ( d ). The percentage of Tie2 + monocytes within in the non-classical monocyte population in individuals with T2DM was increased compared to healthy control individuals, however, without reaching the level of statistical significance (p = 0.056) ( e )
Article Snippet: Of note, CD68 stainings were performed as double labelling combined with the anti-human Tie2 polyclonal goat antibody (1:20, IgG R&D systems AF313), which was detected using peroxidase-labelled rabbit-anti-goat secondary antibody and visualized with Vector® NovaRED® Substrate kit peroxidase.
Techniques: Fluorescence, FACS, Control